Interferon-γ enhances human γδ T cell-mediated osteosarcoma cell killing in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 22-25, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-352316
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the cytotoxic effect of γδ T cells from osteosarcoma patients against interferon-γ (IFN-γ)-treated osteosarcoma cells in vitro.</p><p><b>METHODS</b>Human γδ T cells were amplified by zoledronate from peripheral blood cells of osteosarcoma patients. The expression of Fas on the osteosarcoma cells were measured by flow cytometry and quantitative real-time PCR analysis before and after IFN-γ treatment. The cytotoxicity of γδ T cells against osteosarcoma cells was evaluated with LDH assay.</p><p><b>RESULTS</b>IFN-γ significantly enhanced the susceptibility of the osteosarcoma cell lines HOS and U2OS to the cytotoxicity of γDelta; T cells from osteosarcoma patients (P<0.01). IFN-γ obviously up-regulated the expression of Fas in HOS and U2OS cells (P<0.01). Anti-FasL mAb failed to inhibit the cytotoxicity of γδ T cells in untreated osteosarcoma targets (P>0.05), but significantly impaired γδ T cell cytotoxicity in IFN-γ pre-treated osteosarcoma targets (P<0.01).</p><p><b>CONCLUSION</b>IFN-γ can enhance the cytotoxic effect of human γδ T cells from osteosarcoma patients against osteosarcoma cells in vitro.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Neoplasias Ósseas
/
Linfócitos T Citotóxicos
/
Osteossarcoma
/
Interferon gama
/
Receptores de Antígenos de Linfócitos T gama-delta
/
Receptor fas
/
Biologia Celular
/
Citotoxicidade Imunológica
/
Linhagem Celular Tumoral
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2013
Tipo de documento:
Artigo
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