Enhancement of kallikrein-related peptidase 10 expression attenuates proliferation and invasiveness of human tongue cancer cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1796-1799, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-352332
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of kallikrein-related peptidase 10 (KLK10) on the proliferation and invasiveness of human tongue cancer cell line Tca8113.</p><p><b>METHODS</b>The eukaryotic expression vector harboring KLK10 gene (pIRES2-EGFP-KLK10) was transfected in Tca8113 cells and the stable cell lines were selected by G418 screening. The mRNA and protein expression of KLK10 in transfected Tca8113 cells were assayed by RT-PCR and Western blotting, respectively, and the proliferation and invasiveness of the cells were evaluated by MTS cell growth assay and Transwell chamber invasion experiments.</p><p><b>RESULTS</b>A stable Tca8113 cell line with high KLK10 expression was obtained, which showed significantly increased mRNA and protein expression levels of KLK10 and obviously attenuated proliferation and invasiveness compared with control and empty vector-transfected cells (P<0.05).</p><p><b>CONCLUSION</b>Enhancing KLK10 gene expression can decrease the proliferation and invasiveness of human tongue cancer cells in vitro.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Neoplasias da Língua
/
Calicreínas
/
Regulação Neoplásica da Expressão Gênica
/
Movimento Celular
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Vetores Genéticos
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2012
Tipo de documento:
Artigo
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