High-mobility group box protein 1 in synergy with interleukin-1β promotes interleukin-8 expression in human airway epithelial cells in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 1764-1767, 2012.
Article
em Zh
| WPRIM
| ID: wpr-352339
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To test the effect of high-mobility group box protein 1 (HMGB1) alone or in synergy with interleukin-1β (IL-1β) on the expression of IL-8 in human airway epithelial cells in vitro.</p><p><b>METHODS</b>Human airway epithelial 16HBE and A549 cell lines were incubated with HMGB1 (100 ng/ml) in the absence or presence of IL-1β (10 ng/ml) for 24 h, and the changes of IL-8 mRNA and protein expressions were assessed using quantitative PCR and enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>In the two human airway epithelial cell lines, HMGB1 alone did not produce obvious effect on the expression of IL-8, but in the presence of IL-1β, HMGB1 caused a significant increase of IL-8 expressions at both the mRNA and protein levels.</p><p><b>CONCLUSION</b>HMGB1 in synergy with IL-1β increases the expression of IL-8 in human airway epithelial cells, which provides new evidence that HMGB1 contributes to neutrophilic airway inflammation by regulating IL-8 expression.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Brônquios
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RNA Mensageiro
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Linhagem Celular
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Interleucina-8
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Biologia Celular
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Proteína HMGB1
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Células Epiteliais
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Interleucina-1beta
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Inflamação
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2012
Tipo de documento:
Article