Molecular subtyping of Staphylococcus aureus isolated from a severe food-poisoning / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 672-676, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-352414
ABSTRACT
<p><b>OBJECTIVE</b>To study the molecular types of Staphylococcus aureus isolated from a severe food-poisoning and to trace the possible strains.</p><p><b>METHODS</b>Real-time PCR was applied to detect nuc gene as a specific marker for S. aureus, mecA gene encoding methicillin resistance and 5 other genes encoding staphylococcal enterotoxins (sea, seb, see, sed, see). Isolates were also performed with 16S rRNA oligonucleotide sequence analyzing by DNAStar MegAlign 5.0 software and pulse-field gel electrophoresis (PFGE) by BioNumerics Version 4.0 software.</p><p><b>RESULTS</b>The nuc gene was detected from the 10 isolated strains, sea and seb genes were detected from 7 strains. There were 4 16 S rRNA types and 5 PFGE types found from all the strains.</p><p><b>CONCLUSIONS</b>Three relative S. aureus strains were involved in the severe food-poisoning at least. Molecular subtyping might give a molecular epidemiological evidence and support the source tracing of an outbreak.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Intoxicação Alimentar Estafilocócica
/
Staphylococcus aureus
/
China
/
Epidemiologia
/
Técnicas de Tipagem Bacteriana
/
Classificação
/
Eletroforese em Gel de Campo Pulsado
/
Enterotoxinas
/
Genética
/
Microbiologia
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2008
Tipo de documento:
Artigo
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