An improved method for acute isolation of neurons from the hippocampus of adult rats suitable for patch-clamping study / 生理学报
Acta Physiologica Sinica
; (6): 112-117, 2004.
Article
em En
| WPRIM
| ID: wpr-352805
Biblioteca responsável:
WPRO
ABSTRACT
An improved method is described for fast and reliable isolation of neurons from hippocampus of adult rats by a combination of mechanical and enzymatic means. The procedure allows the isolation of neurons from 500-600-d-old rats (over 300 g), preserving the proximal dendritic structure without impairing the electrical characteristics of the cells. Morphologically distinct neurons can be recognized. Using cell-attached, inside-out and whole-cell configurations of patch clamp technique, it was shown that the enzymatically isolated neurons in hippocampus from rats weighing more than 300 g exhibited voltage-gated calcium, sodium and potassium currents, outwardly rectifying chloride channel and large conductance Ca(2+)-activated potassium channel currents. Approximately, 95% of healthy cells allowed the formation of giga-ohm seals.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fisiologia
/
Ativação do Canal Iônico
/
Separação Celular
/
Ratos Wistar
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Técnicas de Patch-Clamp
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Biologia Celular
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Canais de Potássio Cálcio-Ativados
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Hipocampo
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Métodos
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Acta Physiologica Sinica
Ano de publicação:
2004
Tipo de documento:
Article