Separation and morphological observation of mouse testis seminiferous tubule epithelium / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 629-631, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-352974
ABSTRACT
<p><b>OBJECTIVE</b>To explore a simple and feasible technique to locate proteins during spermatogenesis.</p><p><b>METHODS</b>Various germ cells and somatic cells were separated by collagenase I and DNase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and confocal microscopy.</p><p><b>RESULTS</b>Germ cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus.</p><p><b>CONCLUSION</b>A simple method for locating proteins during spermatogenesis was successfully developed.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Epitélio Seminífero
/
Espermatozoides
/
Separação Celular
/
Células Cultivadas
/
Biologia Celular
/
Métodos
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2011
Tipo de documento:
Artigo
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