Effects of AQP1 overexpression on morphology and water transport in mouse Schwann cells / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 60-64, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-353117
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effect of AQP1 gene on facial nerve edema following injury through investigation of the relationship between the expression of AQP1 gene and Schwann cells swelling.</p><p><b>METHODS</b>The AQP1 expression in Schwann cells of mouse facial nerve tissues was detected by immunofluorescent staining. The transgenic protocol by lentivirus transduction was used to specifically upregulate AQP1 expression in Schwann cells. Lenti-AQP1 and CTRL (empty vector) transduced cells were observed during gene overexpression every 24 h for 6 days by using phase contrast microscopy. Cell volume of CTRL and Lenti-AQP1 treated cells was measured daily from the day of treatment, through day 6.</p><p><b>RESULTS</b>Schwann cell primary cultures maintained a high level of AQP1 water channels, representing an ideal cell model to study the role of AQP1 in the facial nerve. The expression of AQP1 mRNA and protein in Schwann cells infected with the Lenti-AQP1 was increased significantly compared with CTRL lentivirus (P < 0.05). Lenti-AQP1 caused cell swelling in cultured Schwann cells, as validated by cell volume determinations (P < 0.01).</p><p><b>CONCLUSIONS</b>AQP1 is an important factor responsible for the fast water transport of cultured Schwann cells. It plays an important role in facial nerve edema.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células de Schwann
/
Fatores de Tempo
/
Transdução Genética
/
Virologia
/
RNA Mensageiro
/
Regulação para Cima
/
Lentivirus
/
Biologia Celular
/
Tamanho Celular
/
Edema
Tipo de estudo:
Guia de Prática Clínica
/
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2016
Tipo de documento:
Artigo
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