The effect of Metformin on the proliferation and collagen synthesis of human keloids fibroblasts / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 291-295, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-353162
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Metformin on the proliferation and collagen synthesis of the human keloids fibroblasts as well as the effect on phosphorylation of Akt/FoxO1 signal transduction pathway.</p><p><b>METHODS</b>Fibroblasts of keloid were divided into control group treated with medium solution and experimental groups treated with different concentrations of Metformin. 48 h later CCK-8 assay was adopted to evaluate cell survival; Western blot was performed to detect the Akt and FoxO1 phosphorylation; and Hydroxyproline reagent kit was used to detect the collagen synthesis.</p><p><b>RESULTS</b>With different concentrations (30, 60, 90, 120 mmol/L) of Metformin, the absorbance of cultured keloid fibroblasts detected by CCK8 assay decreased by (13.30 ± 2.04)%, (22.64 ± 4.70)%, (54.00 ± 5.34)% and (63.12 ± 3.48)%. The growth of fibroblasts was suppressed by Metformin in a dose-dependent manner. It showed that the level of phoshpo-akt and phoshpo-foxOl in keloids fibroblasts in experimental groups was lower than that in the control group and the collagen synthesis were also decreased in experimental groups, all in a dose-dependent manner (P < 0.05, P < 0.01).</p><p><b>CONCLUSIONS</b>Metformin can effectively inhibit the proliferation and collagen synthesis of the human keloids fibroblasts in vitro, which may be associated with the suppression of phosphorylation of Akt/FoxO1 signaling pathway</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Fosforilação
/
Transdução de Sinais
/
Colágeno
/
Biologia Celular
/
Proliferação de Células
/
Relação Dose-Resposta a Droga
/
Proteínas Proto-Oncogênicas c-akt
/
Fatores de Transcrição Forkhead
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2015
Tipo de documento:
Artigo
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