Inhibition of binding peptides on replication of duck hepatitis B virus / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 116-120, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-353235
ABSTRACT
<p><b>OBJECTIVE</b>To study the inhibitory effect of binding peptides on duck hepatitis B virus (DHBV) replication in duck hepatocytes.</p><p><b>METHODS</b>Specific binding peptides to duck hepatitis B virus polymerase (DHBVP) were screened by phage display technology (PDT), then were sequenced and synthesized. Binding peptides were added into primary culture of duck hepatocytes infected with DHBV in vitro. DHBV-DNA in the cytoplasm, cell nucleus and medium supernatant was assayed over time.</p><p><b>RESULTS</b>Seven binding peptides were obtained after 3-round screening by PDT. Duck primary hepatocytes infected by DHBV were treated with above obtained binding peptides. The DHBV-DNA levels in medium supernatant and cytoplasm of duck hepatocytes treated with synthesized peptides (the 3rd and the 6th peptide) were significantly lower than those of control cells (P<0.05).</p><p><b>CONCLUSION</b>Specific binding peptides to DHBVP could inhibit the replication of DHBV.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Virologia
/
Replicação Viral
/
Vírus da Hepatite B do Pato
/
Células Cultivadas
/
DNA Polimerase Dirigida por RNA
/
Infecções por Hepadnaviridae
/
Ácidos Nucleicos Peptídicos
/
Hepatócitos
/
Patos
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2005
Tipo de documento:
Artigo
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