Effect of HIV Tat protein on CCR5 expression in monocytes and infection with monocyte-tropic HIV strains / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 532-545, 2004.
Article
em Zh
| WPRIM
| ID: wpr-353266
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of HIV Tat protein on CCR5 expression of monocytes and HIV infection in monocytes.</p><p><b>METHODS</b>Membrane expression of CCR5 on monocytes was analyzed by flow cytometry. Stimulated with HIV Tat protein, monocytes were infected with monocyte-tropic HIV(Ba-L) and HIV gag p24 level in the supernatant was measured by ELISA methods.</p><p><b>RESULTS</b>HIV Tat protein increased CCR5 expression in human monocytes,which was inhibited by rabbit anti-Tat polyclonal antibody. Tat protein also increased p24 level after monocyte-tropic HIV-1(Ba-L) infected monocytes.</p><p><b>CONCLUSION</b>Tat increases CCR5 expression and HIV-1 infection in monocytes, which indicates that HIV Tat might be a key protein in HIV-1 infection.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Farmacologia
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Monócitos
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Produtos do Gene tat
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Infecções por HIV
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HIV
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Receptores CCR5
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Produtos do Gene tat do Vírus da Imunodeficiência Humana
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Citometria de Fluxo
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Genética
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2004
Tipo de documento:
Article