Determination of perfluoropropane in canine whole blood by purge and trap concentrator-GC-MS / 药学学报
Acta Pharmaceutica Sinica
;
(12): 358-360, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-353496
ABSTRACT
<p><b>AIM</b>To develop a method for direct determination of perfluoropropane in canine whole blood and to study its pharmacokinetics after a suspension of perfluoropropane-containing albumin microcapsules was administered intravenously.</p><p><b>METHODS</b>Perfluoropropane-containing albumin microcapsule suspension was administered intravenously to anesthetized canines at the dosage of 0.6 mL x kg(-1). Whole blood samples were collected and added directly into the purging glass tube in Tekmar 3000 Purge and Trap Concentrator coupled with a GC-MS for the determination of perfluoropropane. The pharmacokinetic parameters of perfluoropropane were calculated by non-compartment model statistics.</p><p><b>RESULTS</b>The linear range was 0.0168-4.03 mg x L(-1). The main pharmacokinetic parameters of perfluoropropane was obtained as follows mean residence time (MRT) was (63 +/- 5) s, T1/2 was (44 +/- 4) s, Tmax was 30 s, Cmax was (2.20 +/- 0.20) mg x L(-1), AUC0-infinity was (96 +/- 11) mg x s x L(-1).</p><p><b>CONCLUSION</b>The method is sensitive, specific and simple. It can be used to determine fluorocarbon contained in microcapsule ultrasound contrast agents for studying its pharmacokinetics.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Cápsulas
/
Farmacocinética
/
Área Sob a Curva
/
Albuminas
/
Fluorocarbonos
/
Injeções Intravenosas
/
Cromatografia Gasosa-Espectrometria de Massas
/
Métodos
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2005
Tipo de documento:
Artigo
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