PI3-kinase mediates thrombin-induced platelet aggregation through mDia1 pathway / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 176-180, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-353625
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of mDia1 (mammalian diaphanous 1)in platelet and the role of mDia1 or phosphatidylinositol 3-kinase (PI3K) in the process of thrombin-induced platelet aggregation.</p><p><b>METHODS</b>The extent of platelet aggregation was measured by a platelet aggregation system and the expression of mDia1 and its relation with F-actin in quiescent, spreading or aggregated platelets by Western blot.</p><p><b>RESULTS</b>There was no significant difference in mDia1 expression level between quiescent and activated platelets. mDia1 moved from a Triton-X100-soluble cytosolic fraction to insoluble cytoskeleton fraction after thrombin induced platelets aggregation. Anti-mDia1 antibody could inhibit this aggregation. PI3K inhibitor Wortmannin or Ly294002 inhibited the thrombin induced platelet aggregation and the above mentioned mDia1 translocation.</p><p><b>CONCLUSION</b>PI3-kinase mediates the thrombin-induced platelet aggregation through mDia1 pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Plaquetas
/
Inibidores da Agregação Plaquetária
/
Trombina
/
Agregação Plaquetária
/
Actinas
/
Fosfatidilinositol 3-Quinases
/
Metabolismo
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2010
Tipo de documento:
Artigo
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