Cloning of adriamycin-resistant related (arr) gene in an adriamycin-resistant L1210 variant
Experimental & Molecular Medicine
;
: 145-149, 1998.
Artigo
em Inglês
| WPRIM
| ID: wpr-35391
ABSTRACT
A partial fragment of novel sequence (arr, adriamycin-resistant related) was previously identified using the differential display (DD)-PCR technique with adriamycin-resistant L1210 variant (L1210AdR), which shows a typical multidrug resistant (MDR) phenotypes. The present research shows the isolation of full length arr cDNA sequence. To clone the full length cDNA of arr gene, DD-PCR fragments were subjected to 5'- and 3'-Rapid Amplification of cDNA End (RACE) method. The cloned arr cDNA consisted of 770 bases and contained an open reading frame of 153 bases, encoding a protein of 51 amino acid with the molecular mass of 4 kDa by in vitro translation reactions. Northern blot analysis showed that a 770 bases transcript arr gene was overexpressed in adriamycin-resistant L1210 variant, but not in the parent suggesting that the arr gene may be involved in the adriamycin-resistant phenotypes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Biossíntese de Proteínas
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Glicoproteínas
/
Leucemia L1210
/
Doxorrubicina
/
Regulação Neoplásica da Expressão Gênica
/
Northern Blotting
/
Sequência de Aminoácidos
/
Clonagem Molecular
Limite:
Animais
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
1998
Tipo de documento:
Artigo
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