Regulation of epithelial sodium channel α-subunit expression by adenosine receptor A₂a in alveolar epithelial cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1551-1555, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-353946
ABSTRACT
<p><b>BACKGROUND</b>The amiloride-sensitive epithelial sodium channel α-subunit (α-ENaC) is an important factor for alveolar fluid clearance during acute lung injury. The relationship between adenosine receptor A(2a) (A(2a)AR) expressed in alveolar epithelial cells and α-ENaC is poorly understood. We targeted the A(2a)AR in this study to investigate its role in the expression of α-ENaC and in acute lung injury.</p><p><b>METHODS</b>A549 cells were incubated with different concentrations of A(2a)AR agonist CGS-21680 and with 100 µmol/L CGS-21680 for various times. Rats were treated with lipopolysaccharide (LPS) after CGS-21680 was injected. Animals were sacrificed and tissue was harvested for evaluation of lung injury by analysis of the lung wet-to-dry weight ratio, lung permeability and myeloperoxidase activity. RT-PCR and Western blotting were used to determine the mRNA and protein expression levels of α-ENaC in A549 cells and alveolar type II epithelial cells.</p><p><b>RESULTS</b>Both mRNA and protein levels of α-ENaC were markedly higher from 4 hours to 24 hours after exposure to 100 µmol/L CGS-21680. There were significant changes from 0.1 µmol/L to 100 µmol/L CGS-21680, with a positive correlation between increased concentrations of CGS-21680 and expression of α-ENaC. Treatment with CGS-21680 during LPS induced lung injury protected the lung and promoted α-ENaC expression in the alveolar epithelial cells.</p><p><b>CONCLUSION</b>Activation of A(2a)AR has a protective effect during the lung injury, which may be beneficial to the prognosis of acute lung injury.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fenetilaminas
/
Alvéolos Pulmonares
/
Adenosina
/
Linhagem Celular
/
Western Blotting
/
Receptores Purinérgicos P1
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Biologia Celular
/
Canais Epiteliais de Sódio
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2011
Tipo de documento:
Artigo
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