Study on in vitro microdialysis recovery of Shuanghuanglian / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the feasibility of microdialysis techniqiue to be used in pharmacokinetic study of Chinese medicine, taking Shuanghuanglian as a model drug.</p><p><b>METHOD</b>The samples were obtained by retrodialysis, determined by HPLC gradient elution to calculate the in vitro recovery rate (RR) of specific components. To study the difference of RR of a certain component in different dialysis mediums, and the effect of flow rates and concentration on RR.</p><p><b>RESULT</b>Along with the increase of number of substances in the dialysis medium, the RR of specific components reduced. But the RR was independent of the concentration of the component in the dialysis medium. The RR reduced with the increasing flow rate in the same dialysis medium.</p><p><b>CONCLUSION</b>The microdialysis technique can be used in pharmacokinetics study of Chinese medicine.</p>