Influence of LY294002 on protein kinase B expression in salivary adenoid cystic carcinoma (SACC)-83 and SACC-LM cells / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influence of LY294002 on protein kinase B expression in salivary adenoid cystic carcinoma cells (SACC).</p><p><b>METHODS</b>Using Western-blot and RT-PCR detected the protein and mRNA level of protein kinase B (PKB) both in cell line, absent and present LY294002. The data were analysed by t-test.</p><p><b>RESULTS</b>The expression and transcription level of PKB (Ser(473)) in SACC-83 was lower than in SACC-LM (P < 0.05). The expression of PKB (Ser(473)) in SACC cell lines with LY294002 was lower than in SACC cell lines without LY294002 (P < 0.01). The transcription level of PKB showed no difference in SACC cell lines with and without LY294002 (P > 0.05).</p><p><b>CONCLUSIONS</b>In vitro, the protein expression and transcription level of PKB (Ser(473)) is higher in SACC-LM cell line than in SACC-83 cell line. In vitro, LY294002 can inhibit protein expression of PKB (Ser(473)) in SACC-83 cells and SACC-LM cells.</p>