Establishing a high-titer infectious avian influenza A (H5N1) pseudotyped viral particle / 病毒学报
Chinese Journal of Virology
;
(6): 324-329, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-354728
ABSTRACT
A transient four-plasmid cotransfection system was used to construct avian influenza A (H5N1) pseudotyped viral particle (H5N1Pp) by incorporating hemagglutinin (HA) protein and neuraminidase (NA) protein from H5N1 avian influenza virus onto Murine leukemia virus pseudotyped viral particles, the transmission electron microscopy, infectivity titer assay, hemagglutination assay, neutralization assay of H5N1Pp were studied. We established a pseudotyped H5N1 viral particle at a high titer of 10(8) Pp/mL, the morphology,the hemagglutination activity and neutralization specificity of H5N1Pp is simililar to wild H5N1 virus. The research result sets a platform for studying this virus, including its receptors, the functional analysis of HA and NA, neutralizing antibodies and anti-H5N1 drug development.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Vírion
/
Virologia
/
Aves
/
Testes de Neutralização
/
Transfecção
/
Engenharia Genética
/
Glicoproteínas de Hemaglutininação de Vírus da Influenza
/
Carga Viral
/
Virus da Influenza A Subtipo H5N1
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS