Cloning and expression of gp37 gene of avian leukosis virus subgroup J / 病毒学报
Chinese Journal of Virology
;
(6): 178-184, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-354751
ABSTRACT
The transmembrane protein (TM) encoded by gp37 gene plays a critical role when virus fusion with cell membrane occurs. Several highly conserved regions in TM are important targets for antivirus studies. Studies on structure and function of TM will provide basic information for anti-retrovirus, especially for avian leukosis virus. In the study, gp37 gene was amplified by PCR from the Chinese strain ALV-J-WS0701. The gp37 gene was cloned into pMD18-T vector, and was sequenced. Then, pFast-BacHTb-gp37 vector was constructed and expressed by baculovirus expression vector system. The expression product of gp37 gene was analyzed by indirect immunofluorescence assay and Western blot. The results showed that positive green fluorescence was present in sf9 cells infected with recombinant virus and a protein band with a molecular weight of 21kD was present in Western blot. It is concluded that gp37 gene was expressed in sf9 cells infected with recombinant virus successfully.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Leucose Aviária
/
Expressão Gênica
/
Linhagem Celular
/
Galinhas
/
Proteínas do Envelope Viral
/
Classificação
/
Clonagem Molecular
/
Vírus da Leucose Aviária
/
Spodoptera
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2012
Tipo de documento:
Artigo
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