Screening and identification of receptor reacting with nucleocapsid protein of duck enteritis virus / 病毒学报
Chinese Journal of Virology
;
(6): 63-66, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-354770
ABSTRACT
To clarify the pathogenesis of Duck enteritis virus (DEV), the cDNA library of duck's liver infected by DEV and a bait plasmid containing DEV nucleocapsid protein (NP) gene were constructed, then the receptor was screened from the cDNA library plasmid by the yeast two-hybrid system and verified by GST pull-down test. The results showed that the capacity of the primary cDNA library was 1 x 106 CFU with insertion size from 0.5 to 1 kb, and the bait plasmid of pGBKT7-NP showed no self-activation. The receptor reacting with DEV NP in duck liver was initially confirmed as the protein kinase C inhibitor (PKCI). These results provide new clues for further investigation on pathogenesis of DEV.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Receptores Virais
/
Virologia
/
Virulência
/
Biblioteca Gênica
/
Alphaherpesvirinae
/
Proteínas do Nucleocapsídeo
/
Técnicas do Sistema de Duplo-Híbrido
/
Patos
/
Genética
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2012
Tipo de documento:
Artigo
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