A GeXP based multiplex RT-PCR assay for simultaneous detection of twelve human respiratory viruses / 病毒学报
Chinese Journal of Virology
;
(6): 526-532, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-354795
ABSTRACT
A GeXP based multiplex RT-PCR assay was developed to simultaneously detect twelve different respiratory viruses types/subtypes including influenza A virus, influenza B virus, influenza A virus sH1N1, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, human rhinovirus, human metapneumovirus, adenovirus, respiratory syncytial virus A, respiratory syncytial virus B and human bocavirus. Twelve sets of specific primers were designed based on the conserved sequences of available respiratory-virus sequence database. The specificity of the multiplex system was examined by positive specimens confirmed previously. The sensitivity to detect twelve respiratory viruses simultaneously was 10(3) copies/microL. Twenty four clinical specimens were further detected by this novel assay and the results were compared with that of the real-time RT-PCR. These results showed that this novel assay based on GeXP is a fast, sensitive, and high throughput test for the detection of respiratory virus infections.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Orthomyxoviridae
/
Vírus Sinciciais Respiratórios
/
Infecções Respiratórias
/
Rhinovirus
/
Vírus de RNA
/
Virologia
/
Sensibilidade e Especificidade
/
Infecções por Orthomyxoviridae
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus da Influenza A Subtipo H1N1
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2011
Tipo de documento:
Artigo
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