Indomethacin-induced HL-60 cell apoptosis is associated with inhibition of beta-catenin/c-myc signal transduction pathway / 中华血液学杂志
Chinese Journal of Hematology
; (12): 629-631, 2003.
Article
em Zh
| WPRIM
| ID: wpr-354814
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of indomethacin on HL-60 cell proliferation and apoptosis, and elucidate partly the molecular mechanism about the anti-leukemia effect of indomethacin by studying beta-catenin signal transduction pathway.</p><p><b>METHODS</b>HL-60 cells were treated with indomethacin at different concentrations (0, 25, 50, 100, 200, 400 micro mol/L). Cells viability and proliferation were determined by Trypan blue staining and cell counting respectively. DNA ladder pattern and cell morphology were used to identify cell apoptosis. The expression and cleavage of caspase-3, beta-catenin, c-myc were detected by Western blot techniques.</p><p><b>RESULTS</b>Indomethacin could significantly inhibit HL-60 cells proliferation and induce cells apoptosis with a time and dose dependent manner. An up-regulating expression and cleavage of caspase-3 were observed. Indomethacin could inhibit beta-catenin expression and induce its degradation, c-myc protein exhibited a down-regulation with a concentration dependent manner.</p><p><b>CONCLUSION</b>Indomethacin could inhibit HL-60 cell proliferation and induce cells apoptosis. Anti-leukemia effect of indomethacin was associated with the inhibition of "beta-catenin --> c-myc" signal pathway.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Fatores de Tempo
/
Transdução de Sinais
/
Transativadores
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Anti-Inflamatórios não Esteroides
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Indometacina
/
Western Blotting
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Apoptose
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Células HL-60
/
Caspases
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Ano de publicação:
2003
Tipo de documento:
Article