Study on DNA methylation status of WT1 gene promoter in leukemia cell / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyse the WT1 expression and its DNA methylation status of its promoter domain.</p><p><b>METHOD</b>The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.</p><p><b>RESULTS</b>WT1 was overexpressed in HL60, K562 and KG1 leukemia cell lines, but not in U937 and PBMNC. Methylation of WT1 promoter was not observed in HL60 cells.</p><p><b>CONCLUSION</b>DNA methylation of WT1 gene promotor did not inhibit its expression. Other mechanisms may appear to regulate the WT1 expression.</p>