Dendritic cells (DC) induced from acute myeloid leukemia (AML) cells with cytokine cocktails / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 365-368, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-354859
ABSTRACT
<p><b>OBJECTIVES</b>To explore the feasibility of DC being in vitro induced from AML cells with cytokine cocktails and their biological properties.</p><p><b>METHODS</b>AML cells were cultured in either presence or absence of cytokine cocktails. DC were studied for morphology, and cytochemical and immunofluorescent staining. Functions of DC were examined by MLC, FITC-conjugated dextran uptake test, and LDH release assay. RT-PCR and FISH were used to analyze the specific fusion genes of culture-derived DC.</p><p><b>RESULTS</b>Classical DC morphological changes occurred in all 15 cultured AML cells. DC-associated surface molecules such as CD(1a), CD(80), CD(86), CD(106), CD(83) and HLA-DR were upregulated (P < 0.05). The allostimulatory abilities of culture-derived DC were significantly higher than those of AML cells uncultured or cultured in the absence of cytokines (P < 0.05). Culture-derived DC only in the presence of GM-CSF + IL-4 have phagocytotic activities. CTL assay was performed in 5 of the 15 samples. At effector/target ratio of 201, auto-T lymphocytes primed with the culture-derived DC exhibited no more killing activity to auto-AML cells than those stimulated by IL-2 or uncultured AML cells. Culture-derived DC presenced the native AML-specific aberrant karyotype and related fusion gene.</p><p><b>CONCLUSIONS</b>Cytokine cocktails could in vitro induce AML cells into DC with classical morphology, immunophenotype and function. DC maturity induced by different cytokine cocktails could be variable. Culture-derived DC were originated from the native AML cells. AML cells could make the auto-T lymphocyte anergy.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Técnicas In Vitro
/
Células Dendríticas
/
Monócitos
/
Leucemia Mieloide Aguda
/
Diferenciação Celular
/
Citocinas
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Interleucina-4
Limite:
Adolescente
/
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2003
Tipo de documento:
Artigo
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