Survivin antisense RNA enhances taxol-induced apoptosis in leukemia cell line HL-60 / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 351-354, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-354863
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of survivin antisense RNA on taxol-induced apoptosis in leukemia cell line HL-60.</p><p><b>METHODS</b>A survivin antisense eukaryotic vector pcDNA3-SVVas was transferred into HL-60 cells by electroporation. The live fraction was determined by trypan blue dye exclusion assay. Cell counting and MTT assay were performed to evaluate the sensibility of the transfected cells to taxol. Apoptosis was detected by DNA gel electrophoresis and nuclear staining.</p><p><b>RESULTS</b>Two positive cell clones, HL-60 SVVas and HL-60 neo were obtained. Compared to HL-60 and HL-60 neo cells, HL-60 SVVas cells growth was significantly reduced (P < 0.05). By MTT assay, the IC(50) of taxol to HL-60 SVVas, HL-60 neo and HL-60 cells were (14.4 +/- 1.87) ng/ml, (31.9 +/- 6.38) ng/ml and (32.0 +/- 3.52) ng/ml, respectively, the difference was significant by statistic analysis (P < 0.01). Agarose gel electrophoresis of genomic DNA from HL-60 SVVas showed typical DNA ladder, but DNA from HL-60 neo and HL-60 did not. Nuclei become condense in HL-60 SVVas cells.</p><p><b>CONCLUSION</b>Survivin antisense RNA could enhance taxol-induced apoptosis in leukemia cell line HL-60. This may lay an experimental foundation for further research of gene therapy in leukemia.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Transfecção
/
RNA Antissenso
/
Paclitaxel
/
Apoptose
/
Células HL-60
/
Sinergismo Farmacológico
/
Proteínas Inibidoras de Apoptose
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Genética
/
Proteínas Associadas aos Microtúbulos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2003
Tipo de documento:
Artigo
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