Transfection of chk1/2 antisense oligonucleotide to HL-60 cell line increases the apoptotic sensitivity to irradiation / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 253-255, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-354877
ABSTRACT
<p><b>OBJECTIVE</b>To block signal transduction of cell cycle checkpoints by antisense blocking of chk1/2 gene to increase the radiation sensitivity of HL-60 cell line.</p><p><b>METHOD</b>To transfect the HL-60 cell with chk1/2 antisense and sense chain alone and in combination, expose the cells to irradiation at 24 h after the transfection, the chk1 protein change was assayed by Western blot and the cell cycles and annexin V apoptosis rates by FCM.</p><p><b>RESULTS</b>The irradiated apoptosis sensitivity was increased by antisense blocking of chk1 gene in HL-60 cell line, the apoptotic rate was 26.31% being significantly higher than that of the sense blocking (10.34%) (P < 0.05), Furthermore, the G(2)/M phase blocking phenomenon decreased and a synergic effect was observed in antisense blocking both the chk1 and chk2 genes.</p><p><b>CONCLUSION</b>Antisense blocking of chk1/chk2 could increase the apoptotic sensitivity to irradiation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Quinases
/
Efeitos da Radiação
/
Tolerância a Radiação
/
Transfecção
/
Ciclo Celular
/
Oligonucleotídeos Antissenso
/
Proteínas Serina-Treonina Quinases
/
Apoptose
/
Células HL-60
/
Quinase do Ponto de Checagem 2
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2003
Tipo de documento:
Artigo
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