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Effect of endogenous TGF-beta1 and TNF-alpha on the As(2)O(3) inducing apoptosis of HL-60 cells / 中华血液学杂志
Chinese Journal of Hematology ; (12): 231-234, 2003.
Article em Zh | WPRIM | ID: wpr-354883
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of endogenous TGF-beta(1) and TNF-alpha on As(2)O(3) inducing apoptosis of HL-60 cells.</p><p><b>METHODS</b>The expressions of endogenous TGF-beta(1) and TNF-alpha in apoptotic HL-60 cells induced by As(2)O(3) were assayed by RT-PCR, quantitative RT-PCR, ELISA, DNA fragmentation and TUNEL. The effect of TGF-beta(1) and TNF-alpha antisense phosphorothioate oligodeoxynucleotides (PSODNs) on As(2)O(3) inducing apoptotic HL-60 cells was further studied.</p><p><b>RESULTS</b>(1) Expressions of endogenous TGF-beta(1) and TNF-alpha were significantly up-regulated in As(2)O(3) inducing apoptotic HL-60 cells (from 13,546 +/- 124 and 497,216 +/- 187 before treatment to 23,273 +/- 229 and 674,217 +/- 189 after treatment, respectively), accompanied with down-regulated bcl-2 mRNA expression (from 10,424 +/- 274 before treatment to 3,361 +/- 89 after treatment). (2) TGF-beta(1) and TNF-alpha antisense PSODNs could rescue As(2)O(3) induced apoptosis of HL-60 cells, with a restoration of bcl-2 gene expression.</p><p><b>CONCLUSIONS</b>Endogenous TGF-beta(1) and TNF-alpha played an important role in As(2)O(3) inducing HL-60 cells apoptosis through down-regulation of bcl-2 expression.</p>
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: Óxidos / Farmacologia / Fisiologia / Arsenicais / RNA Mensageiro / Regulação para Baixo / Fator de Necrose Tumoral alfa / Apoptose / Receptores do Fator de Necrose Tumoral / Células HL-60 Limite: Humans Idioma: Zh Revista: Chinese Journal of Hematology Ano de publicação: 2003 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Óxidos / Farmacologia / Fisiologia / Arsenicais / RNA Mensageiro / Regulação para Baixo / Fator de Necrose Tumoral alfa / Apoptose / Receptores do Fator de Necrose Tumoral / Células HL-60 Limite: Humans Idioma: Zh Revista: Chinese Journal of Hematology Ano de publicação: 2003 Tipo de documento: Article