Study of the inhibitory effect of NF-kappaB decoy on tissue factor gene expression and FVII activation in cultured human umbilical vein endothelial cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 149-151, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-354898
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of NF-kappaB decoy on tissue factor (TF) expression and FVII activation in cultured human umbilical vein endothelial cells (HUVEC), and to explore new methods for prevention and treatment of coronary heart disease.</p><p><b>METHODS</b>NF-kappaB decoy transfection efficiency was detected by flow cytometry, NF-kappaB decoy's mechanism was analyzed by electrophoretic mobility shift assay (EMSA), TF mRNA was detected by RT-PCR, TF antigen expression on the surface of HUVEC by flow cytometry, FVIIa level in plasma incubated with HUVEC stimulated by TNF-alpha by rsTF one stage clotting method.</p><p><b>RESULTS</b>NF-kappaB decoy could be successfully transfected into HUVEC. It could compete with the endogenous kappaB cis sequence element in the regulatory regions of TF promoter to bind transcriptional factor NF-kappaB. It could also significantly inhibit the TF mRNA, TF antigen expression on the cell surface and TF function leading to activation of FVII.</p><p><b>CONCLUSION</b>NF-kappaB decoy could inhibit TF gene expression and FVII activation in cultured HUVEC and might be a potential new strategy for prevention and treatment of coronary heart disease.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligodesoxirribonucleotídeos
/
Farmacologia
/
Veias Umbilicais
/
Fator VII
/
RNA Mensageiro
/
Tromboplastina
/
Transfecção
/
Células Cultivadas
/
Fator de Necrose Tumoral alfa
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2003
Tipo de documento:
Artigo
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