Expension In Vitro of T Cells from Cord Blood CD34(+) Cells Stimulated with SCF and IL-2 / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 48-51, 2000.
Article
em Zh
| WPRIM
| ID: wpr-354909
Biblioteca responsável:
WPRO
ABSTRACT
The generation of large quantities of novel human T cell clones ex vivo would make a wide range of gene-and immuno-therapies for tumor and AIDS possibly. Although it is well established that T cells are derived from CD34(+) cells, the involvement of thymic fragments from either human or murine fetus makes the in vitro T cell perliferation very cumbersome. In this report, cord blood mononuclear cells were used as accessory cells to support the differentiation of CD34(+) cells into naive T cells stimulated with SCF and IL-2. CD4(+) and CD8(+) T cells, under the cultural conditions, were continuously produced in vitro at least over a period of 3 weeks and their ratios changed gradually. CD4/CD8 double positive T cells and RAG-2 gene were existed, and RAG-2 gene, reponsible for TCR rearrangement, was expressed during the cell proliferation. Our study presents a simple culture system in vitro to acquire large quantities of naive T cell clones.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2000
Tipo de documento:
Article