Preliminary Study on the Effects of Novel Retinoids SX-115 and CHU-012 on NB4 Cell Line / 中国实验血液学杂志

ABSTRACT

In this research, the effects of two new retinoids, SX-115 and CHU-012, on promyelocytic leukemia cell line NB4 were studied in vitro. Cell proliferation, cell morphologic characters, cell cycle kinetics, reduction ability of NBT, differentiation antigens, immunofluorescence staining and RT-PCR were adopted as the observational parameters. The results showed that SX-115 and CHU-012 induced differentiation of NB4 cells at concentration of 10(-6) mol/L. Comparing the effects of the two retinoids with all trans-retinoic acid (ATRA) at same concentration, there was no significant difference among the three agents. The mechanism of the 2 new retinoids remains possibly the same as ATRA.