Effect of non-contact coculture on bone marrow stromal cells and neural stem cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 823-826, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-355012
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of non-contact coculture on bone marrow stromal cells (MSCs) and neural stem cells (NSCs) in neural stem cell culture medium.</p><p><b>METHODS</b>MSCs and NSCs were cultured in non-contact coculture in Transwell plate, and cell morphology and immunocytochemical profile were investigated.</p><p><b>RESULTS</b>In the coculture, the NSCs showed adhering growth and extended long processes, and the migrating cells formed a network of cells within 7 days. The cells differentiated into neurons, astrocytes and oligodendrocytes as shown by immunocytochemistry. Most of the MSCs grew in a non-adherent manner, giving rise to large spherical cell masses which expressed neuronal, astrocyte, and oligodendrocyte phenotypes. In the control group, the NSCs grew in suspension, some of MSCs formed small non-adherent spherical cell masses, while some cells showed adherent growth.</p><p><b>CONCLUSION</b>MSCs and NSCs in the non-contact coculture can mutually promote the cell differentiation into neural cells in neural stem cell culture medium, indicating that both MSCs and NSCs can secrete some neurotrophic factors to provide a microenvironment suitable for survival and differentiation for each other.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Células da Medula Óssea
/
Diferenciação Celular
/
Células Cultivadas
/
Células Estromais
/
Ratos Wistar
/
Técnicas de Cocultura
/
Biologia Celular
/
Células-Tronco Neurais
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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