Effect of hTERT antisense oligodeoxynucleotide on telomerase activity in bladder cancer cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 779-781, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-355019
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of hTERT antisense oligodeoxynucleotide on telomerase activity in bladder cancer cells.</p><p><b>METHODS</b>Antisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and purified. Telomerase activity was measured by telomerase PCR ELISA kit. hTERT mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR), and hTERT protein by immunohistochemistry and flow cytometry.</p><p><b>RESULTS</b>Telomerase activity was decreased in T24 cells 48 h after treatment with AS PS-ODN, and was significantly inhibited at 72 h.</p><p><b>CONCLUSION</b>AS PS-ODN can significantly inhibit telomerase activity by down-regulating hTERT mRNA and protein expression in bladder cancer cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Neoplasias da Bexiga Urinária
/
RNA Mensageiro
/
Regulação Neoplásica da Expressão Gênica
/
Telomerase
/
Oligodesoxirribonucleotídeos Antissenso
/
Linhagem Celular Tumoral
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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