Effect of liposome-mediated HSV-tk gene transfer on fibroblast apoptosis in rats with scald injury / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effect of HSV-tk gene transfer on the apoptosis of fibroblast in rats with scald injury.</p><p><b>METHODS</b>The recombinant eukaryotic expression vector pcDNA3.1/tk was transfected via liposome in the skin of rats with scald injury. The expression of tk gene was detected by RT-PCR technique, and after GCV injection, the apoptosis of the fibroblasts positive for tk gene was observed under transmission electron microscope.</p><p><b>RESULTS</b>Liposome-mediated HSV-tk gene transfection of the rat skin resulted in the positive expression of tk gene in the fibroblasts in the burn wound. GCV injection induced the apoptosis of the positively transfected fibroblasts.</p><p><b>CONCLUSION</b>HSV-tk gene transfer mediated by liposome can promote the apoptosis of the fibroblasts in rats with scald injury.</p>