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Positional cloning of a novel allele of zebrafish cloche mutant / 南方医科大学学报
Journal of Southern Medical University ; (12): 458-462, 2010.
Artigo em Chinês | WPRIM | ID: wpr-355101
ABSTRACT
<p><b>OBJECTIVE</b>To perform the genetic identification of cloche(172) mutant zebrafish.</p><p><b>METHODS</b>The chemical mutagen N-ethyl-N-nitrosourea (ENU) was used to treat the AB stain male fish. Large-scale forward genetic screening was carried out to search for lyC-deficient zebrafish mutant by WISH. The morphology changes of the embryos at 3 days postfertilization (3dpf) stage were observed and the cloche(172) gene was identified by mapping and complementation test.</p><p><b>RESULTS</b>We selected 4 lyC-deficient zebrafish by WISH. cloche(172) mutant showed morphological changes similar to cloche mutant in 3dpf stage. One fourth of the embryos showed cloche phenotype as found in complementation test, and the cloche(172) gene was mapped on the telomere of zebrafish 13 chromosome where cloche gene was located. Numerous red blood cells were observed in the cloche(172) mutant, while only a few cells were found in the cloche mutant in the tail region by o-dianisdine staining.</p><p><b>CONCLUSION</b>cloche(172) gene which is responsible for the phenotype of cloche mutant may be a novel point mutation allele of the cloche mutant.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peixe-Zebra / Muramidase / Embriologia / Mapeamento Cromossômico / Clonagem Molecular / Proteínas de Peixe-Zebra / Embrião não Mamífero / Alelos / Etilnitrosoureia / Toxicidade Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Peixe-Zebra / Muramidase / Embriologia / Mapeamento Cromossômico / Clonagem Molecular / Proteínas de Peixe-Zebra / Embrião não Mamífero / Alelos / Etilnitrosoureia / Toxicidade Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2010 Tipo de documento: Artigo