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Cytotoxic effect of cisplatin with proteasome inhibitor on osteosarcoma cells / 浙江大学学报·医学版
Article em Zh | WPRIM | ID: wpr-355196
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the cytotoxic effect of cisplatin with proteasome inhibitor on osteosarcoma cells.</p><p><b>METHODS</b>Cell survival was tested by MTT, apoptotic morphology was observed by electron microscopy, apoptotic rate was analyzed by flow cytometry, the transcription level of excision repair cross complementation-1 (ERCC-1) was tested by reverse transcription polymerase reaction.</p><p><b>RESULTS</b>Compared with cells treated with cisplatin alone, cells treated with cisplatin and proteasome inhibitor showed a decreased survival rate, more typical apoptotic morphology, higher apoptotic rate [(14.37 +/-2.37)% vs. (50.93 +/-4.84)%, P<0.01)], and lower transcription level of excision repair cross complementation-1.</p><p><b>CONCLUSION</b>Proteasome inhibitor could increase the cytotoxic effect of cisplatin on osteosarcoma cells and promote cisplatin-induced osteosarcoma apoptosis. These effects may be associated with the decreased transcription of excision repair cross complementation-1.</p>
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: Oligopeptídeos / Patologia / Farmacologia / Neoplasias Ósseas / Células Tumorais Cultivadas / Osteossarcoma / Cisplatino / Apoptose / Sinergismo Farmacológico / Antineoplásicos Limite: Animals Idioma: Zh Revista: Zhejiang Daxue xuebao. Yixue ban Ano de publicação: 2005 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Oligopeptídeos / Patologia / Farmacologia / Neoplasias Ósseas / Células Tumorais Cultivadas / Osteossarcoma / Cisplatino / Apoptose / Sinergismo Farmacológico / Antineoplásicos Limite: Animals Idioma: Zh Revista: Zhejiang Daxue xuebao. Yixue ban Ano de publicação: 2005 Tipo de documento: Article