Purification and anti-cancer activity of ricin / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 217-219, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-355238
ABSTRACT
<p><b>OBJECTIVE</b>To extract and purify ricin from castor beans and to evaluate its anti-cancer activity.</p><p><b>METHODS</b>Ricin was purified from castor beans according the modified method of Nicolson and Blaustin. The lectins were extracted in 0.01 mol/L phosphate buffered saline and isolated in the 40% to 80% fraction of ammonium sulfate precipitation. The dialyzed fractionated preparation was applied with a Sepharose 4B column. The lectins were eluted with a linear lactose gradient (0.01 mol/L approximately 0.5 mol/L). Ricin was separated from the ricinus agglutinin by gel filtration on a Sephadex G-100. MTT was applied to analyze the cytotoxicity with different dosage of ricin in different cancer cell lines.</p><p><b>RESULTS</b>There was no difference between the killing effect of normal cells and that of colon cancer cells by using the high dosage of ricin (5 x 10(-8) mol/L approximately 5 x 10(-10) mol/L). However, the cytotoxicity was significant different in those cells with the low dosage of ricin (5 x 10(-11) mol/L approximately 5 x 10(-13) mol/L). Meanwhile ricin had the similar cytotoxicity to leukemia cell K562 and colon cancer cell SW480.</p><p><b>CONCLUSION</b>Ricin is able to kill tumor cells selectively at low concentration, but the selectivity does not appear at high concentrations.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Ricina
/
Linfócitos T Citotóxicos
/
Neoplasias Colorretais
/
Ratos Sprague-Dawley
/
Células K562
/
Linhagem Celular Tumoral
/
Relação Dose-Resposta a Droga
/
Antineoplásicos Fitogênicos
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2005
Tipo de documento:
Artigo
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