Ganoderic acid A suppresses proliferation and invasion and induces apoptosis in human osteosarcoma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 619-624, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-355314
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of ganoderic acid A (GA-A) on the biological behaviors of human osteosarcoma cells in vitro.</p><p><b>METHODS</b>MG63 and HOS cells were treated with 0.1, 0.25, and 0.5 mmol/L GA-A, and the changes in cell proliferation, apoptosis and migration were evaluated using MTT assay, flow cytometry, and Transwell assay, respectively. The expressions of STAT3, p38, and NF-κB1 in the cells were analyzed by Western blotting.</p><p><b>RESULTS</b>GA-A effectively inhibited the proliferation of human osteosarcoma HOS and MG-63 cells in a dose-dependent manner, and induced obvious cell apoptosis in both cells. Treatment with 0.5 mmol/L GA-A also resulted in significant inhibition of the invasion of both cells. The results of Western blotting showed that GA-A down-regulated the expression level of phosphorylated STAT3 and increased the phosphorylation level of p38 and NF-κB1 expression in both cells.</p><p><b>CONCLUSION</b>GA-A can induce proliferation inhibition, apoptosis and suppression of invasion in human osteosarcoma HOS and MG-63 cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Fosforilação
/
Neoplasias Ósseas
/
Osteossarcoma
/
Movimento Celular
/
Apoptose
/
Linhagem Celular Tumoral
/
Proteínas Quinases p38 Ativadas por Mitógeno
/
Proliferação de Células
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2015
Tipo de documento:
Artigo
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