Immunophenotypic characterization of normal peripheral blood B lymphocyte by flow cytometry: reference for diagnosis of chronic B cell leukemia/lymphoma / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 398-404, 2003.
Article
em En
| WPRIM
| ID: wpr-355637
Biblioteca responsável:
WPRO
ABSTRACT
To establish reference values of various immunophenotypic markers in B lymphocyte population in healthy Chinese adults and build background information for accurate interpretation of B cell immunophenotyping data in clinical practice, peripheral blood from 41 healthy adults were collected separately into test tubes containing EDTA-K(2) and stored in room temperature no more than 24 hours before analysis. Whole blood lysis technique and multiparameter flow cytometry were applied to immunophenotype B cells gated on CD19/SSC dot-plot. The results showed that CD22, CD20, CD62L, CD40, CD24, CD79b, CD79a, and FMC-7 were almost positive in the circulating B cell population, whereas CD11a, CD80, CD103, CD10, CD40L, CD54, CD95L, CD86, and CD95 were almost negative in the peripheral blood B lymphocytes. CD18, CD44, CD23, CD5, CD11c and CD43 were positive in different B cell subpopulations. 78% of B cells were IgD positive and ratio kappa/lambda was 1.26. The significance of all these markers in the differential diagnosis of lymphoproliferative diseases was discussed. The conclusion is that it is necessary to consider the qualitative and quantitative levels of expression of various markers in normal B cell population in order to accurately interpret the pathological immunophenotypic data in clinical practice. It is also important to note the immunotypic differences of B cells between Chinese and Western populations.
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Índice:
WPRIM
Assunto principal:
Linfócitos B
/
Leucemia Linfocítica Crônica de Células B
/
Imunofenotipagem
/
Receptores de IgE
/
Molécula 1 de Adesão Intercelular
/
Antígenos CD5
/
Alergia e Imunologia
/
Citometria de Fluxo
/
Linfoma
/
Métodos
Tipo de estudo:
Diagnostic_studies
/
Qualitative_research
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Journal of Experimental Hematology
Ano de publicação:
2003
Tipo de documento:
Article