Development of the human/rat chimera model with neonatal rats / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 297-300, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-355660
ABSTRACT
The purpose of this study was to transplant neonatal rat with human cord blood Lin(-) cells to test the possibility of this xenograft model. The Lin(-) cells were purified from human cord blood (CB) using negative selection strategy based on different lineage-specific antigens. The Lin(-) cells were injected into the liver of neonatal rats using a microinjector at an average of 5 x 10(5) cells for each. Peripheral blood (PB) and spleen were collected at 2,4 and 8 weeks after injection. Flow cytometry was performed to detect human cells in the rat PB, PCR was used to detect human cells in PB as well as spleen. The results showed that a definite proportion of human cells existed in peripheral blood of chimeric rat and the human specific beta2 microglobulin gene fragments were detected in spleen genomic DNA of chimeric rat. It is concluded that human/rat chimera model can be developed with neonatal rats. Human/rat xenograft model may provide a useful and convenient method for human hematopoietic stem cell assay in vivo.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Baço
/
Transplante Heterólogo
/
Sangue
/
DNA
/
Reação em Cadeia da Polimerase
/
Microglobulina beta-2
/
Ratos Sprague-Dawley
/
Antígenos Comuns de Leucócito
/
Quimeras de Transplante
/
Transplante de Células-Tronco de Sangue do Cordão Umbilical
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2003
Tipo de documento:
Artigo
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