Advance in Reseach on Human Immunodeficiency Virus Type I Vector / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 75-78, 2000.
Article
em Zh
| WPRIM
| ID: wpr-355729
Biblioteca responsável:
WPRO
ABSTRACT
Human immunodeficiency virus type-I (HIV-I), one kind of lentiviruses, was characterized by a complex genome that encodes two regulatory proteins and four accessory proteins in addition to the common gag, pol and env gene products. So far, a few of different types of replication-defective vectors were constructed, the highest viral titer from one of which was above 10(7) TU/ml. Several studies on packaging cell line found that eliminating the four accessory genes would have few effect on transduction ability of vector and split-genome package system could reduce the possibility of producing replication-competent virus. There are two kinds of characters on HIV-I vectors. Firstly, it was highly efficient in transducing CD34(+) human hematopoietic stem/progenitor cells; secondly, repeated injections of the HIV-I vector into animal did not elicit the rejection response. HIV-I vector had an extensive host range.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2000
Tipo de documento:
Article