Effects of hepatocyte growth factor gene transfer in combination with echocardiographic destruction of microbubbles in a rat model of experimental myocardial infarction / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 936-939, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-355861
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate whether ultrasonic microbubble destruction (US/MB) could enhance the therapeutic effects of hepatocyte growth factor (HGF) gene transfer for acute myocardial infarction (MI).</p><p><b>METHODS</b>MI was induced by left anterior descending artery ligation in male SD rats. Two to 4 hours thereafter, MI rats were randomly treated with tail vein infusing pc-DNA3.1-HGF plasmid mixed with microbubbles (US/MB-HGF group, n = 18); tail vein infusing pc-DNA3.1-HGF plasmid mixed with saline (US-HGF group, n = 18); tail vein infusing empty plasmid mixed with microbubbles (US/MB-P group, n = 18). All rats were exposed to ultrasound treatment thereafter till contrast imaging disappeared in cardiac region. Rats were sacrificed at 24 hours, 7 days or 14 days, respectively (n = 6 each) and myocardial protein expression of bcl-2 and HGF as well as microvascular density (MVD) were determined.</p><p><b>RESULTS</b>The myocardial protein expressions of bcl-2 and HGF in US/MB-HGF group were significantly higher than those in US-HGF and US/MB-P groups at 7 days post MI (all P < 0.01) and MVD was significantly higher in US/MB-HGF group (367.6 +/- 17.6) than that in US-HGF (268.9 +/- 0.8) and US/MB-P (186.8 +/- 11.8) groups (all P < 0.05) at 14 days post MI.</p><p><b>CONCLUSIONS</b>Ultrasound-mediated microbubble destruction could enhance systemic HGF administration induced myocardial angiogenesis and reduce systemic HGF administration induced myocardial apoptosis in rats with acute MI.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Terapêutica
/
Terapia Genética
/
Ratos Sprague-Dawley
/
Fator de Crescimento de Hepatócito
/
Neovascularização Fisiológica
/
Microbolhas
/
Infarto do Miocárdio
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2008
Tipo de documento:
Artigo
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