Your browser doesn't support javascript.
loading
Plasma metabonomics study of ischemic cerebral apoplexy rats treated with Tongsaimai pellets / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1028-1033, 2012.
Artigo em Chinês | WPRIM | ID: wpr-356022
ABSTRACT
<p><b>OBJECTIVE</b>To observe abnormal metabolic changes caused by ischemic cerebral apoplexy and the regulating action of Tongsaimai pellets on abnormal metabolism by analyzing the change of small molecules in plasma of ischemic cerebral apoplexy rat. To find the potential biomarkers, and to explore metabolic mechanisms of Tongsaimai pellets.</p><p><b>METHOD</b>Rat models of middle cerebral artery occlusion was established with electric coagulation, and rats were divided into 4 groups, model group, sham-operation group, Tongsaimai pellets group and positive control group. Tongsaimai pellets and positive control group were orally administrated by 13.2 g x kg(-1) x d(-1) of crude drugs and 32 mg x kg(-1) x d(-1) of Nimodipine respectively, m odel and sham-operation group by equal volume of distilled water for a week. Plasma of model and sham-operation group were collected, and plasma of Tongsaimai pellets and positive control group were collected on the 1st, 3rd , 7th day after administration. Endogenous metabolites of four groups were determined with GC-MS. Partial least squares discriminant analysis (PLS-DA) was applied to analyze multivariate data and set up model, and T-test was used in significant statistical analysis.</p><p><b>RESULT</b>Compared with sham-operation group rats, pyruvic acid, taurine and hydroxyproline obviously increased in model group rats, while lactic acid, glyceric acid, aminomalonic acid, fructose, tryptophan and leucine significantly decreased, so these metabolites were potential metabolic biomarkers. These endogenous metabolites except taurine got restoration in Tongsaimai group rats.</p><p><b>CONCLUSION</b>Abnormal metabolite level in plasma can be certainly recovered by Tongsaimai pellets, and the treatment of Tongsaimai pellets can be connected with the regulation of related metabolic pathways.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Taurina / Triptofano / Sangue / Medicamentos de Ervas Chinesas / Isquemia Encefálica / Ratos Sprague-Dawley / Ácido Láctico / Ácido Pirúvico / Acidente Vascular Cerebral / Usos Terapêuticos Limite: Animais Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2012 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Taurina / Triptofano / Sangue / Medicamentos de Ervas Chinesas / Isquemia Encefálica / Ratos Sprague-Dawley / Ácido Láctico / Ácido Pirúvico / Acidente Vascular Cerebral / Usos Terapêuticos Limite: Animais Idioma: Chinês Revista: China Journal of Chinese Materia Medica Ano de publicação: 2012 Tipo de documento: Artigo