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Effect of L-Arg on inflammatory reaction and nuclear factor-kappa B signal pathway in the acute lung injury in rats induced by lipopolysaccaride / 中国应用生理学杂志
Chinese Journal of Applied Physiology ; (6): 90-93, 2010.
Artigo em Chinês | WPRIM | ID: wpr-356208
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects and the mechanisms of L-Arginine (L-Arg), the critical substrate for nitric oxide (NO) production, on pulmonary inflammatory cytokine expression and Nuclear Factor-kappa B signal pathway in a model of lipopolysaccharide (LPS) induced acute lung injury (ALI).</p><p><b>METHODS</b>Model of ALI was induced by injection (iv) with LPS 5 mg/kg in male SD rats. L-Arg (500 mg/kg ip) was administrated at 3 h or 6 h after LPS injection respectively for 3 h, and the rats were killed at 6 h or 9 h after saline (control) or LPS injection. The expression and the translocation of NF-kappa B P65 in lung tissue were detected with immunohistochemisty (IHC). The gene expression of intercellular adhesion molecule-1 (ICAM-1) was examined by RT-PCR. The concentrations of TNF-alpha and IL-6 in lung tissue were respectively evaluated by radioimmunoassay. The pathological changes of lung tissue were observed by light microscope.</p><p><b>RESULTS</b>Compared with LPS group, treatment with L-Arg at 3 h after LPS significantly decreased the expression of NF-kappa B protein. The concentrations of TNF-alpha and IL-6 in lung tissue were significantly decreased and the lung damage was inproved respectively compared with that of the LPS (3 h + 3 h) group. The lung damage was alleviated in L-Arg (3 h + 3 h) group.</p><p><b>CONCLUSION</b>Relatively early administration of L-Arg might protect lung from LPS-induced injury by inhibiting NF-kappa B activation and subsequently inhibiting the NF-kappa B-mediated release of inflammatory factors.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Arginina / Transdução de Sinais / Lipopolissacarídeos / NF-kappa B / Interleucina-6 / Fator de Necrose Tumoral alfa / Ratos Sprague-Dawley / Usos Terapêuticos / Lesão Pulmonar Aguda Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Arginina / Transdução de Sinais / Lipopolissacarídeos / NF-kappa B / Interleucina-6 / Fator de Necrose Tumoral alfa / Ratos Sprague-Dawley / Usos Terapêuticos / Lesão Pulmonar Aguda Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2010 Tipo de documento: Artigo