The study in primary cultured astrocytes following fluid percussion injury / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 46-50, 2010.
Article
em Zh
| WPRIM
| ID: wpr-356220
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the morphology alterations and proteomics changes in primary astrocytes following fluid percussion injury.</p><p><b>METHODS</b>Primary cultures of astrocytes were prepared from cerebral hemispheres of 1-3 d-old SD rats, then, astrocytes were randomly divided into control group and injury group which were subjected to (0.2 +/- 0.01) MPa fluid percussion injury. The changes of protein expression pattern in astrocytes between injury and control groups were monitored with two dimensional gel electrophoresis.</p><p><b>RESULTS</b>Astrocytes' s abnonmalities of morphology after injury were apparent. The fluid percussion injury caused astrocytes edema, shrinkage, cell junction disconnection and necrosis at 2 h after injury. 24 h and 48 h after injury, most part of astrocytes's dendrites and soma became hypertrophy and showed a higher rate of cell proliferation. The dynamic proteomics changes were identified and total different 13 spots were detected in this study from the 2DE gels. The different displayed 5 spots were identified via MALDI-TOF: cofilin 1, destrin, phosphoglycerate mutase 1, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10, annexin 1.</p><p><b>CONCLUSION</b>The obvious alteration of morphology and protein expression pattern in primary cultured astrocytes could be induced after fluid percussion injury. The differential proteins detected were probably related to stress responses.</p>
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Índice:
WPRIM
Assunto principal:
Fisiologia
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Estresse Fisiológico
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Lesões Encefálicas
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Proteínas
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Córtex Cerebral
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Astrócitos
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Ratos Sprague-Dawley
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Proteoma
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Biologia Celular
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Proteômica
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2010
Tipo de documento:
Article