Electrophysiological characteristics of the open state of neuronal alpha4beta2, alpha4beta4 and alpha7-nicotinic acetylcholine receptors / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 392-396, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-356243
ABSTRACT
<p><b>AIM</b>To establish the whole-cell recording techniques of the neuronal alpha4beta2, alpha4beta4, and alpha7-nicotinic acetylcholine receptors heterologously expressed in SH-EP1 cell line and discuss the electrophysiological characteristics of their open states.</p><p><b>METHODS</b>The cells were cultured with DEME medium(high glucose) and suitable for electrophysiological experiments three days after passage. The receptors were induced from resting states into open states by rapid application of nicotine (alpha4beta2, alpha4beta4) or choline (alpha7).</p><p><b>RESULTS</b>The SH-EP1 cells cultured by this method were in good conditions and expressed plenty of receptors. Alpha4beta2, alph4beta4 and alpha7 inward currents could be induced by rapid application of agonists but had different dynamic processes against time. All the three types of currents were dose and voltage-dependent and had inward rectification property.</p><p><b>CONCLUSION</b>The open states of neuronal alpha4beta2, alpha4beta4, and alpha7-nicotinic acetylcholine receptors and their transitions have distinct characteristics and the inward currents of all this three types of receptors are dose and voltage-dependent and have inward rectification property.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Encéfalo
/
Transfecção
/
Linhagem Celular
/
Receptores Nicotínicos
/
Técnicas de Patch-Clamp
/
Biologia Celular
/
Células Epiteliais
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Receptor Nicotínico de Acetilcolina alfa7
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Potenciais da Membrana
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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