Effects of human HSF1 on transcriptional regulation of prohibitin promoter / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 349-352, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-356256
ABSTRACT
<p><b>AIM</b>To assay the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.</p><p><b>METHODS</b>The total length of hHSF1 exon was amplified by PCR method and cloned into pcDNA3. 1(+) vector. pcDNA3. 1(+)-hHSF1 and pGI3 prohibitin were co-transfected into HEK293 cells. The luciferase activity was detected by Dual-Luciferase Reporter Assay System to evaluate the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.</p><p><b>RESULTS</b>The pcDNA3.1(+)-hHSF1 vector was constructed successfully. The assay of luciferase activity showed that the transcription of pGL3-prohibitin was dramatically upregulated by hHSF1.</p><p><b>CONCLUSION</b>hHSF1 can transcriptionally regulate prohibitin expression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Proteínas Repressoras
/
Fatores de Transcrição
/
Transcrição Gênica
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Transfecção
/
Regulação da Expressão Gênica
/
Regiões Promotoras Genéticas
/
Proteínas de Ligação a DNA
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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