The proliferation profile of mouse spermatoganial stem cells in three types of culture media / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To establish an in vitro long-term culture system of mouse Spermatogonial stem cells (SSCs).</p><p><b>METHODS</b>Three types of serum-free culture media, namely, DMEM/F12, KSR (KnockoutM Serum Replacement) and StemPro-34 SFM, to which the same growth factors including GDNF, soluble GFRalpha1 and bFGF were added equally, and MEF(mouse embryonic fibroblast) feeder layer were used to culture mouse SSCs enriched from pup mice testes through differential adherence selection. The activity of stem cells was examined morphologically, and the marker gene expression of SSCs was detected by RT-PCR and immunocytochemical analysis.</p><p><b>RESULTS</b>The activity of SSCs cultured in DMEM/F12 and KSR serum-free media was only maintained for 6-7 days. However, the StemPro-34 SFM medium could maintain the proliferation of cultured SSCs nearly one month.</p><p><b>CONCLUSION</b>StemPro-34 SFM serum-free medium sustains the proliferation of mouse SSCs in vitro.</p>