The inhibition of tamoxifen on sodium channel in SHG-44 glioma cell-line / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 207-210, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-356295
ABSTRACT
<p><b>AIM</b>To explore the effect of tamoxifen on voltage-dependent sodium channels in SHG-44 glioma cell line.</p><p><b>METHODS</b>Whole-cell patch clamp technique was used to record the Na currents in SHG-44 cell line and to investigate the effect of tamoxifen of different concentration on this channel currents.</p><p><b>RESULTS</b>This channel activated and inactivated quickly. Tamoxifen could significantly decrease the amplitude of Na currents of SHG-44 cell line. This block effect was dose dependent and voltage dependent. When the holding potential was 0 mV, 8 micromol/L tamoxifen could block this currents 69%. The half inhibition concentration (IC50) was 5.54 micromol/L.</p><p><b>CONCLUSION</b>Tamoxifen could significantly block the voltage dependent sodium channel in malignant glioma cell line SHG-44. It might be one of the mechanisms that tamoxifen inhibit glioma proliferation. clamp technique was used to record the Na currents in SHG-44 cell line and to investigate the effect of tamoxifen of different concentration on this channel currents.</p><p><b>RESULTS</b>This channel activated and inactivated quickly. Tamoxifen could significantly decrease the amplitude of Na currents of SHG-44 cell line. This block effect was dose dependent and voltage dependent. When the holding potential was 0 mV, 8 micromol/L tamoxifen could block this currents 69%. The half inhibition concentration (IC50) was 5.54 micromol/L.</p><p><b>CONCLUSION</b>Tamoxifen could signifi-cantly block the voltage dependent sodium channel in malignant glioma cell line SHG-44. It might be one of the mechanisms that tamoxifen inhibit glioma proliferation.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Tamoxifeno
/
Neoplasias Encefálicas
/
Técnicas de Patch-Clamp
/
Antineoplásicos Hormonais
/
Bloqueadores dos Canais de Sódio
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Glioma
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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