Platelet derived growth factor receptor β over-expression in endothelial progenitor cells promote reendothelialization after vascular injury / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 214-218, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-356407
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of platelet derived growth factor receptor β (PDGFR-β) transfected endothelial progenitor cells (EPCs) on vascular regeneration.</p><p><b>METHODS</b>Spleen-derived mononuclear cells (MNCs) were isolated using density gradient centrifugation and induced with special culture medium. EPCs transfection was performed with Lipofectamine(TM) 2000 reagent according to the instruction manual. Carotid artery injury was induced in splenectomized mice. EPCs were injected by tail vein immediately and at 24 h after endothelial injury of the carotid artery. Evans blue staining was performed to evaluate reendothelialization at 7 days after endothelial injury of the carotid artery.</p><p><b>RESULTS</b>Most adherent cells were LDL and UEA-I double positive. Laser scanning confocal microscopy showed that transfection efficiency was about 50%-60%. The reendothelialized area in the PDGFR-β-EPCs group was significantly larger than that in EGFP-EPCs group.</p><p><b>CONCLUSION</b>Transplantation of PDGFR-β over-expressed EPCs can promote reendothelialization in the early phase after carotid artery injury.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Regeneração
/
Células-Tronco
/
Cirurgia Geral
/
Transplante
/
Endotélio Vascular
/
Células Cultivadas
/
Receptores do Fator de Crescimento Derivado de Plaquetas
/
Lesões das Artérias Carótidas
/
Biologia Celular
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2014
Tipo de documento:
Artigo
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