An improved protocol for primary culture of cardiomyocyte from neonatal rat / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 53-56, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-356439
ABSTRACT
<p><b>OBJECTIVE</b>The present study describes an improved in vitro culture method for obtaining high purity, vital and fully functional cardiomyocytes from neonatal rat.</p><p><b>METHODS</b>After cutting ventricular tissue with improved method, ventricular tissues were digested with low concentrations of trypsin overnight at 4 °C, and then underwent collagenase II digestion. Thereafter, cardiomyocytes were purified by combined differential adhesion and chemical inhibition methods.</p><p><b>RESULTS</b>Adherent cardiomyocytes were seen at 12 h after culture, spontaneously beating cardiomyocytes were observed at 24 h after culture, crosslinked cardiomyocytes were found at 48 h after culture, adhesion clustered cardiomyocytes were seen at 72 h after culture, dense network formed from inter-connected was evidenced together with radial arranged cell clusters and cell pseudopodia 96 h the mutual contact woven into and formed radically ordering cell clusters and island-like beating cardiomyocytes at 96 h after culture. The cell survival rate and purity were more than 98%.</p><p><b>CONCLUSION</b>Fully functional spontaneous beating cardiomyocytes can be obtained by the use of this improved primary neonatal rat cardiomyocytes culture method.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células Cultivadas
/
Ratos Sprague-Dawley
/
Biologia Celular
/
Miócitos Cardíacos
/
Cultura Primária de Células
/
Animais Recém-Nascidos
/
Métodos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS