Selective depletion of donor alloreactive T cells by using immuno-magnetic cell sorting / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 637-640, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-356498
ABSTRACT
This study was aimed to explore a new method of alleviating graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation through selective elimination of human alloreactive T cells expressing either CD25(+) or CD69(+) by immuno-magnetic cell sorting (MACS). Healthy donor peripheral blood mononuclear cells were cocultivated with bone marrow mononuclear cells from HLA-nonidentical leukemia recipient with remission in one-way mixed lymphocyte culture (OWMLC). After 3 days, both CD25(+) and CD69(+) lymphocytes were removed by MACS. The depleted donor fraction and untreated donor cells were then rechallenged in a secondary mixed lymphocyte culture (MLC) with the original stimulator cells or a third party to assess relative alloreactivity. The results showed that 50% inhibition of the secondary MLC was observed in the depleted donor fraction. Alloreactivity against unrelated third-party cells was largely preserved. It is concluded that this method reduces alloreactivity while retaining reactivity against a third party target in vitro.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Linfócitos T
/
Antígenos de Diferenciação de Linfócitos T
/
Antígenos CD
/
Transplante de Medula Óssea
/
Separação Imunomagnética
/
Biologia Celular
/
Lectinas Tipo C
/
Alergia e Imunologia
/
Subunidade alfa de Receptor de Interleucina-2
/
Citometria de Fluxo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Artigo
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